Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: The validation of recombinant adenovirus BMP9 and siBMP9. ( A ) The expression of BMP9 in normal bladder mucosa ( n = 126), superficial bladder cancer ( n = 68), and infiltrating bladder cancer ( n = 62) in the Lee Bladder database. p = 0.007; ( B ) The expression levels of BMP9 in T24 and BIU-87 cells were detected by western blot; ( C ) The BMP9 was up-regulated in BIU-87 cells after being transfected with AdBMP9 compared to the control group; ( D ) The BMP9 was down-regulated in T24 cells after being transfected with AdsiBMP9 compared to the control group. Data are shown as mean ± SD. ** p < 0.01.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Biomarker Discovery, Recombinant, Expressing, Western Blot, Transfection, Control

Journal: International Journal of Molecular Sciences

Article Title: BMP9 Promotes the Proliferation and Migration of Bladder Cancer Cells through Up-Regulating lncRNA UCA1

doi: 10.3390/ijms19041116

Figure Lengend Snippet: BMP9 up-regulated the expression of lncRNA UCA1 in bladder cancer cells. ( A ) Five common lncRNA were screened in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( B ) The expression of lncRNA UCA1 were verified in BIU-87 cells after transfected with AdBMP9 by RT-PCR; ( C ) The expression of lncRNA UCA1 were tested in T24 cells after being transfected with AdsiBMP9 by RT-PCR; ( D ) The inhibitory effect of siUCA1 were analyzed by RT-PCR in BIU-87 cells after being co-transfected with AdBMP9 and siUCA1. Data are shown as mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, vs. control groups.

Article Snippet: Human bladder cancer BIU-87 and T24 cells were obtained from the China Center for Type Culture Collection (CCTCC).

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Control

Journal: International Journal of Nanomedicine

Article Title: Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity

doi: 10.2147/IJN.S217277

Figure Lengend Snippet: Primers Sequences Applied in the Procedure of Comparing microRNA Distinguished Expressed Between T24 and RT4 Bladder Cancer Cells

Article Snippet: The original T24 cells and RT4 cells were purchased from Procell Life Science & Technology (Wuhan, China, Lot # CL-0227 and # CL-0431).

Techniques: Sequencing

Journal: International Journal of Nanomedicine

Article Title: Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity

doi: 10.2147/IJN.S217277

Figure Lengend Snippet: MiR-155 sustains the proliferation rates of T24 cells and promotes the proliferation rates of RT4 cells assessed by CCK-8 assay. Notes: T24 group represents T24 cells; miR155-inhibitor-T24 group represents T24 cells transfected with miR-155 inhibitors; inhibitor-NC-T24 group represents T24 cells transfected with miR-155 inhibitor-NCs; miR155-inhibitor-T24+miR155-mimic group represents T24 cells transfected by miR155-inhibitors and followed by miR155-mimics transfection; miR155-inhibitor-T24+mimic-NC group represents T24 cells transfected by miR-155-inhibitors and followed by miR155-mimic-NCs transfection. The RT4 group represents RT4 cells; miR155-mimic-RT4 group represents RT4 cells transfected by miR155-mimics; mimic-NC-RT4 group represents RT4 cells transfected by miR155-mimic-NCs; miR155-mimic-RT4+miR155-inhibitor group represents RT4 cells transfected by miR-155-mimics and followed by miR-155 inhibitor transfection; miR155-mimic-RT4+inhibitor-NC group represents RT4 cells transfected with miR-155-mimics and followed by miR-155 inhibitor-NCs transfection. Cells in the miR155-inhibitor-T24 group showed a lower proliferation rate compared to the original T24 group ( A ). Cells in the miR155-mimic-RT4 group showed a higher proliferation rate compared to the original RT4 group ( B ). ** p < 0.001. Abbreviation: CCK-8, Cell counting kit-8.

Article Snippet: The original T24 cells and RT4 cells were purchased from Procell Life Science & Technology (Wuhan, China, Lot # CL-0227 and # CL-0431).

Techniques: CCK-8 Assay, Transfection, Cell Counting

Journal: International Journal of Nanomedicine

Article Title: Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity

doi: 10.2147/IJN.S217277

Figure Lengend Snippet: MiR-155 induced cell cycle re-distribution in T24 and RT4 cells. Notes: A, B, C, D and E represent T24 group, miR155-inhibitor-T24 group, inhibitor-NC-T24 group, miR155-inhibitor-T24+miR155-mimic group and miR155-inhibitor-T24+mimic-NC group. F, G, H, I and J represent RT4 group, miR155-mimic-RT4 group, mimic-NC-RT4 group, miR155-mimic-RT4+miR155-inhibitor group and miR155-mimic-RT4+inhibitor-NC group. Compared to the T24 group, more cells were arrested in G0-G1 phase, and less cells were in the S phase in the miR155-inhibitor-T24 group ( K ). Cells in miR155-mimic-RT4 group accumulated in the S phase and decreased in G0-G1 phase compared to the original RT4 group ( L ). ** p < 0.001.

Article Snippet: The original T24 cells and RT4 cells were purchased from Procell Life Science & Technology (Wuhan, China, Lot # CL-0227 and # CL-0431).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity

doi: 10.2147/IJN.S217277

Figure Lengend Snippet: MiR-155 sustains the invasive ability of T24 cells and promotes the invasive ability of RT4 cells assessed by Transwell assay. Notes: A, B, C, D and E represent T24 group, miR155-inhibitor-T24 group, inhibitor-NC-T24 group, miR155-inhibitor-T24+miR155-mimic group and miR155-inhibitor-T24+mimic-NC group. F, G, H, I and J represent RT4 group, miR155-mimic-RT4 group, mimic-NC-RT4 group, miR155-mimic-RT4+miR155-inhibitor group and miR155-mimic-RT4+inhibitor-NC group. Cell invasive abilities in the miR155-inhibitor-T24 group were decreased compared to the original T24 group ( K ). Also, cell invasive abilities in the miR155-mimic-RT4 group were significantly increased compared to the original RT4 group ( L ). Bar = 50 μm. ** p < 0.001.

Article Snippet: The original T24 cells and RT4 cells were purchased from Procell Life Science & Technology (Wuhan, China, Lot # CL-0227 and # CL-0431).

Techniques: Transwell Assay

Journal: International Journal of Nanomedicine

Article Title: Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity

doi: 10.2147/IJN.S217277

Figure Lengend Snippet: Micro-structures of TNTs between T24 and RT4 cells observed under SEM. Notes: TNTs ( A , Black arrows) were observed between T24 and RT4 cells. Continuous, membranous, micro-tubular connection between T24 and RT4 cells are shown ( B, C and D ). The TNTs structures were fragile ( D ). The diameter of TNTs ranged from 200 to 500 nm, and the lengths of TNTs between T24 and RT4 cells ranged from 20 μm to 1 mm. Abbreviations: TNTs, tunneling nanotubes; SEM, scanning electron microscopic.

Article Snippet: The original T24 cells and RT4 cells were purchased from Procell Life Science & Technology (Wuhan, China, Lot # CL-0227 and # CL-0431).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Tunneling Nanotubes Mediated microRNA-155 Intercellular Transportation Promotes Bladder Cancer Cells' Invasive and Proliferative Capacity

doi: 10.2147/IJN.S217277

Figure Lengend Snippet: Intercellular transportation of miR-155 from T24 cells to RT4 cells via TNTs. Notes: RT4 cells were labeled by CFSE ( A , Green), and co-cultured with T24 cells for 24 hrs. MiR-155 was labeled by the fluorescent in situ hybridization probe ( B , Red). F-actin was labeled by Phalloidin-iFluor™ 405 Conjugate ( C , Blue). ( A, B and C) were merged as ( D ). Spontaneous migration of miR-155 ( B and D , White arrows) from T24 to RT4 cells via TNTs ( C and D ) was observed by capturing “double positive” (Red and Green) RT4 cells under fluorescence microscopy ( D ). Bar = 10 μm. Abbreviation: miR-155, microRNA-155.

Article Snippet: The original T24 cells and RT4 cells were purchased from Procell Life Science & Technology (Wuhan, China, Lot # CL-0227 and # CL-0431).

Techniques: Labeling, Cell Culture, In Situ Hybridization, Migration, Fluorescence, Microscopy